Therapeutic effects of the as-synthesized polylactic acid/chitosan nanofibers decorated with amphotricin B for in vitro treatment of Leishmaniasis

Leishmaniasis is one of the biggest concerns World Health Organization (WHO) due to its severe side effects, emergence resistance, and secondary bacterial infections; therefore, it was reported as an epidemic, especially in immunocompromised individuals. The main purpose this study synthesize characterize polylactic acid (PLA)/chitosan nanofibers decorated with amphotericin B a new delivery system for vitro treatment leishmaniasis wounds. prepared were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), X-ray diffraction (XRD), drug release test. anti-leishmanial effect against Leishmania major promastigotes cytotoxicity macrophages determined by flow cytometry assay indicated that 84% loaded released after 400 min. average concentration PLA/chitosan conventional form prevented growth 50% L. 1.29 4.34 µg/mL, respectively.